Description
🖤 Activated Charcoal (TC Grade) — high-surface-area adsorption agent (500–1500 m²/g); removes inhibitory compounds from medium and spent metabolites secreted by cultures. Essential for orchid PLB development, rooting of some woody species, and somatic embryo germination.
| 🧪 CAS | 7440-44-0 | 📦 Form | Black powder |
| 🔬 Grade | TC / Adsorption | 🌡️ Storage | RT, sealed, dry |
🖤 What activated charcoal does in TC
Charcoal adsorbs: phenolics (browning compounds), ethylene (gaseous inhibitor that accumulates in sealed containers), 5-hydroxymethylfurfural (autoclaving artefact), spent hormone metabolites, and possibly low levels of exogenous inhibitors in crude agar. This “clean medium effect” dramatically improves: orchid PLB development, somatic embryo germination, and rooting of recalcitrant woody species.
🖤 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Orchid PLB development & maturation | 0.5–2 g/L | Removes ethylene; promotes differentiation |
| Rooting of recalcitrant woody species | 1–3 g/L | WPM + IBA + charcoal is classic |
| SE germination / conversion medium | 1–2 g/L | Removes inhibitory compounds |
| Callus with browning problem | 0.5–1 g/L | Adsorbs phenolic exudates |
| Banana Stage III (rooting) | 1–2 g/L | Widely used in commercial production |
⚠️ Activated charcoal also adsorbs plant growth regulators — especially IAA, GA₃, and zeatin. Do not use charcoal-containing medium when precise hormone concentrations are needed. BAP and IBA are less affected, but test your specific hormone.
Reviews
There are no reviews yet.