Description
🌿 Indole-3-Acetic Acid (IAA) — the primary natural auxin in plants. Less stable and less potent than NAA or IBA in TC conditions, but essential for studying natural hormone signalling and in specific embryogenesis protocols.
| 🧪 CAS | 87-51-4 | ⚖️ MW | 175.18 g/mol |
| 🔬 Grade | TC / Research | 🌡️ Storage | −20 °C, strictly light-protected |
⚠️ Stability warning
⚠️ IAA degrades rapidly in light (photodegradation) and is metabolised by IAA oxidase in plant cells. Wrap stock solutions in foil. Autoclave decomposes IAA — add by filter-sterilisation after autoclaving the basal medium. Effective half-life in culture medium: 12–24 hours under standard light conditions.
🌱 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Rooting (less effective than IBA) | 1–3 mg/L | Use only when protocol specifies IAA |
| Callus maintenance (low level) | 0.01–0.1 mg/L | Background auxin tone |
| Somatic embryogenesis (with cytokinin) | 1–10 mg/L | Some carrot/alfalfa protocols |
| Hormone signalling studies | 0.01–100 µM | Define concentration precisely |
🧪 Stock prep
Dissolve in 70% ethanol (easiest). Do not autoclave — filter-sterilise through 0.22 µm membrane and add to cooled autoclaved medium. Store stock in foil-wrapped tube at −20 °C.
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