Description
🌱 Adenine Sulphate — a purine base that acts as a weak cytokinin synergist; historically important and still used in some shoot proliferation protocols, particularly for potato, carnation, and orchids.
| 🧪 CAS | 321-30-2 | ⚖️ MW | 232.24 g/mol |
| 🔬 Grade | TC / Biochemical | 🌡️ Storage | RT, dry, sealed |
💡 Adenine as a cytokinin synergist
Adenine was identified as a shoot-promoting compound by Skoog & Miller in the 1950s — the work that led directly to the discovery of kinetin. Adenine itself has weak cytokinin activity (via conversion to adenine riboside and interaction with cytokinin receptors) but acts primarily by amplifying the effect of added cytokinins (BAP, zeatin) — particularly useful in protocols where BAP alone gives poor shoot induction.
🌱 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Shoot proliferation synergist | 40–80 mg/L alongside BAP | Potato, carnation, orchid |
| Orchid establishment medium | 20–40 mg/L | With Knudson C or V&W |
| Callus to shoot transition | 40–160 mg/L | Classic tobacco-type protocols |
| Potato (Solanum) micropropagation | 40 mg/L + 0.5 mg/L BAP | Established protocol |
💡 Adenine sulphate is not a substitute for a proper cytokinin — it enhances cytokinin response. If your protocol includes adenine but BAP alone seems insufficient, try adenine + BAP together before increasing BAP concentration.
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