Description
🍌 Banana Multiplication Medium (Stage II) — ready-formulated medium for commercial Musa (banana) micropropagation; pre-optimised with high BAP and the right macro:micro ratio for vigorous shoot multiplication.
| 📦 Format | Powder → 1 L | 🔬 Grade | TC / Commercial |
| ⚗️ Working pH | 5.7 | 🌡️ Storage | 2–8 °C, sealed |
🍌 Why banana has a dedicated medium
Commercial banana TC operates at scale — millions of plants per year. Banana shoots require high BAP (3–7 mg/L) and a specific sucrose:nitrogen balance for fast multiplication without vitrification. Pre-formulated media eliminate the weighing errors that devastate a production batch at commercial scale.
🧫 Stage guide
| Use | Concentration | Notes |
|---|---|---|
| Stage I — Establishment | MS + 3 mg/L BAP + 0.5 mg/L NAA | Sword sucker explant |
| Stage II — Multiplication | This medium (high BAP) | Subculture every 4–6 weeks |
| Stage III — Rooting | ½ MS + 0.5–1 mg/L IBA, no cytokinin | Root induction 3–4 weeks |
| Deflasking / Acclimatisation | ½ MS or plain water agar | Harden before soil transfer |
💡 High BAP banana cultures often show “cigar-shaped” shoots — normal. They elongate rapidly after transfer to hormone-free ½ MS rooting medium.
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