Description
🌿 L-Glutamine — the most important organic nitrogen source in TC; a major amino acid nitrogen carrier and the primary substrate for nitrogen assimilation in plant cells.
| 🧪 CAS | 56-85-9 | ⚖️ MW | 146.15 g/mol |
| 🔬 Grade | TC / Biochemical | 🌡️ Storage | −20 °C; unstable at RT in solution |
💡 Why glutamine over inorganic nitrogen only
L-Glutamine provides directly available reduced organic nitrogen for protein and nucleotide synthesis without the energy cost of NO₃⁻ reduction. It is the primary nitrogen assimilation product (glutamine synthetase generates glutamine from glutamate + NH₄⁺), and is particularly critical in: embryo development (major seed storage nitrogen form), pollen culture, and protoplast culture where high nitrogen demand exists.
🌿 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Embryo rescue / embryo culture | 250–750 mg/L | Replace some NH₄NO₃ |
| Pollen / anther culture | 100–500 mg/L | N&N or B5 base |
| Protoplast culture | 100–500 mg/L | Especially in first 7 days |
| High-growth suspension culture | 100–500 mg/L | Supplement to inorganic N |
⚠️ L-Glutamine decomposes at autoclave temperatures — always filter-sterilise (0.22 µm) and add to cooled autoclaved medium. Glutamine in solution is also unstable at RT — add fresh to medium and use within the same session.
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