Description
🌿 Zeatin — the most important natural cytokinin in plants, first isolated from maize. More potent than BAP or kinetin for many difficult-to-culture species; expensive but irreplaceable for some protocols.
| 🧪 CAS | 13114-27-7 | ⚖️ MW | 219.24 g/mol |
| 🔬 Grade | TC / Research | 🌡️ Storage | −20 °C, light-protected |
⚗️ Why zeatin when you have BAP?
Zeatin is the natural trans-zeatin riboside precursor — plants recognise it as the “real thing” through cytokinin receptors, with higher receptor affinity than BAP in many species. Critical for: orchids (where BAP causes leaf abnormalities), strawberry (where BAP causes fasciation), and many recalcitrant species where synthetic cytokinins produce poor quality shoots.
🌸 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Orchid shoot differentiation | 0.5–5 mg/L | Preferred over BAP for Phalaenopsis |
| Strawberry micropropagation | 0.1–0.5 mg/L | BAP causes fasciation — use zeatin |
| Recalcitrant dicot shoot proliferation | 0.1–2 mg/L | Higher quality than BAP |
| Meristem maintenance (high-value) | 0.05–0.2 mg/L | Where BAP causes problems |
🧪 Stock prep
Dissolve in a few drops of 1M NaOH with gentle warming, then dilute. Zeatin has low water solubility in neutral/acidic conditions. Filter-sterilise. Store at −20 °C in the dark — zeatin degrades faster than BAP.
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