Description
🌲 Woody Plant Medium (WPM) — developed by Lloyd & McCown (1980) at University of Wisconsin; the first medium designed specifically for temperate deciduous trees and shrubs.
| 📦 Format | Powder → 500 mL or 1 L | 🔬 Grade | TC / Tissue Culture |
| ⚗️ Working pH | 5.6–5.8 | 🌡️ Storage | 2–8 °C, sealed |
💡 Low salt = happy woody plants
WPM has ~30% the nitrogen of MS and much lower potassium and phosphate. Woody plants adapted to nutrient-poor forest soils are stunted and necrotic on high-salt media — WPM matches their natural mineral environment, resulting in better shoot elongation, less hyperhydricity, and stronger root systems.
🌲 Best for
| Use | Concentration | Notes |
|---|---|---|
| Apple (Malus) | WPM + 1–2 mg/L BAP | Commercial standard |
| Poplar / Aspen | WPM + 0.1–0.5 mg/L BA | Fast multiplication |
| Oak (Quercus) | WPM + TDZ 0.02–0.1 mg/L | Hard to culture on MS |
| Hazelnut (Corylus) | WPM + 1 mg/L BAP + 0.01 mg/L IBA | |
| Rhododendron (alternative to Anderson’s) | WPM pH 5.0 | Lower nutrient level helps |
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