Description
⚗️ Sodium Hydroxide (NaOH) Pellets — the most-used pH adjustment reagent in TC; also the preferred solvent for sparingly-soluble PGRs like BAP, NAA, zeatin, and 2,4-D.
| 🧪 CAS | 1310-73-2 | ⚖️ MW | 40.00 g/mol |
| 🔬 Grade | TC / Laboratory | 🌡️ Storage | RT, tightly sealed — hygroscopic |
⚗️ Uses
| Use | Concentration | Notes |
|---|---|---|
| Medium pH adjustment (alkalise) | 1M NaOH, dropwise to target pH 5.7–5.8 | Add before autoclaving |
| BAP / NAA / 2,4-D dissolution | Few drops 1M NaOH per 10 mg PGR | Then dilute to water |
| Zeatin dissolution | 1M NaOH with gentle warm | Zeatin has lowest water solubility |
| Ascorbic acid stock (antioxidant) | Dissolve in dilute NaOH for stability | Neutral pH extends shelf life |
💡 Prepare 1M NaOH: dissolve 4.0 g NaOH pellets in 100 mL distilled water. Store in a sealed polypropylene bottle — NaOH absorbs CO₂ from air and forms Na₂CO₃ if left open, which causes pH drift.
⚠️ NaOH pellets are highly hygroscopic and corrosive. Wear gloves when handling. Dissolution is exothermic — add slowly to water, not water to pellets.
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