Description
✨ Silver Nitrate (AgNO₃) — ethylene action inhibitor used to improve shoot quality in transformation and in vitro cultures. Silver ions (Ag⁺) block ethylene perception, suppressing senescence, leaf yellowing, and ethylene-induced inhibition of regeneration.
| 🧪 CAS | 7761-88-8 | ⚖️ MW | 169.87 g/mol |
| 🔬 Grade | TC / Analytical | 🌡️ Storage | Dark, sealed — photosensitive; cool storage |
✨ How Ag⁺ blocks ethylene
Ag⁺ ions bind the copper co-factor in ethylene receptors (ETR1/ERS1), permanently blocking ethylene binding. No ethylene perception → no ethylene response: cultures maintain vigour, leaves stay green, senescence is delayed, and regeneration efficiency improves. Particularly important in transformation protocols where Agrobacterium co-cultivation triggers host ethylene stress responses.
✨ Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Shoot regeneration improvement | 1–10 mg/L | Reduces leaf senescence |
| Transformation media (anti-ethylene) | 2–5 mg/L | Selection and co-cultivation phases |
| Cucumber / cucurbit shoot improvement | 5–10 mg/L | Classic application |
| Anti-ethylene in senescence studies | 1–25 µM (0.17–4.3 mg/L) | Dose-response first |
⚠️ Silver nitrate is photosensitive — solutions turn black (Ag⁰) on exposure to light. Prepare and store in foil-wrapped containers. Also note: AgNO₃ stains skin and clothing permanently. Wear gloves at all times.
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