Description
🌱 ½ MS (Half-Strength MS) — standard MS macronutrients diluted to 50%, keeping vitamins & micronutrients full-strength. The go-to rooting and seed germination medium.
| 📦 Format | Powder → 1 L | 🔬 Grade | TC / Tissue Culture |
| ⚗️ Working pH | 5.7–5.8 | 🌡️ Storage | 2–8 °C, sealed |
💡 When to use ½ MS instead of full MS
High salt stress inhibits root elongation. Cutting the macronutrients in half — especially nitrate and ammonium — reduces osmotic load and encourages root initiation and elongation in shoots transferred from Stage II. Also ideal for sterile seed germination where seedlings need gentle nutrition.
🌱 Best applications
| Use | Concentration | Notes |
|---|---|---|
| Root induction (Stage III) | ½ MS + 0.5–2 mg/L IBA | Low or no cytokinin |
| Arabidopsis seed germination | ½ MS + 1% sucrose | No hormones needed |
| Orchid deflasking | ½ MS → plain half MS | Acclimatise before transfer |
| Embryo rescue | ½ MS + coconut water | Gentle support |
💡 After 3–4 weeks on ½ MS rooting medium, rooted shoots transfer to soil with >90% survival in most species.
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