Description
🌿 L-Proline — a cyclic amino acid with unique roles in stress tolerance, cell wall extensin synthesis, and pollen development. A critical supplement in anther culture and somatic embryogenesis for cereals.
| 🧪 CAS | 147-85-3 | ⚖️ MW | 115.13 g/mol |
| 🔬 Grade | TC / Biochemical | 🌡️ Storage | RT, sealed, dry |
💡 Why proline in TC?
Proline serves three roles in TC: (1) osmotic adjustment — proline accumulation is a universal stress response; exogenous proline improves culture recovery under osmotic stress; (2) pollen wall synthesis — sporopollenin and pollen wall proteins are proline-rich; (3) somatic embryogenesis — proline induces embryogenic competence in some cereals (wheat, barley) and legumes by activating stress-related SE pathways.
🌾 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Cereal anther culture (SE induction) | 100–500 mg/L | Wheat, barley protocols |
| Pollen germination medium | 100–300 mg/L | Improves germination % |
| Somatic embryogenesis (legumes) | 100–500 mg/L | Soybean, alfalfa |
| Osmotic stress tolerance experiments | 50–500 mg/L | Dose-response |
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