Description
🌱 Kinetin (6-Furfurylaminopurine) — the first cytokinin ever identified (Skoog & Miller, 1955), found in aged herring sperm DNA. Historically foundational; still useful as a lower-activity alternative to BAP.
| 🧪 CAS | 525-79-1 | ⚖️ MW | 215.21 g/mol |
| 🔬 Grade | TC / Research | 🌡️ Storage | RT, dark, dry; stock at 4 °C |
⚗️ Kinetin vs BAP
Kinetin has lower cytokinin activity than BAP — you typically need 2–5× higher concentration for the same effect. This can be an advantage: lower risk of vitrification and epigenetic instability over extended subculture. Kinetin is also used to reduce shoot abnormalities by alternating with BAP in long-running cultures.
🌱 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Shoot proliferation (general) | 1–5 mg/L | Higher conc than BAP needed |
| Tobacco & Nicotiana callus | 0.2–2 mg/L | Classic Skoog & Miller system |
| Callus maintenance | 0.1–1 mg/L | With low auxin |
| Alternating with BAP (anti-vitrification) | 2–5 mg/L every other subculture | Reduces long-term abnormalities |
| Fern gametophyte proliferation | 0.5–2 mg/L | Some fern protocols |
🧪 Stock prep
Dissolve in a few drops of 1M HCl or DMSO, then dilute with water. Kinetin is poorly soluble in neutral water and NaOH is less effective than for BAP. Filter-sterilise, store at 4 °C up to 6 months.
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