Description
🥛 Casein Hydrolysate (Casamino Acids) — enzymatic hydrolysate of milk casein; a complex mixture of all 20 amino acids and short peptides. The classic organic nitrogen supplement when inorganic nitrogen alone is insufficient.
| 📦 Form | Powder, off-white | 🔬 Grade | TC / Microbiological |
| ⚗️ N content | ~8–10% total N | 🌡️ Storage | RT, sealed, dry |
💡 When to use casein hydrolysate
Casein hydrolysate provides immediately available reduced organic nitrogen — plants take up amino acids directly via specific transporters without the energy cost of NO₃⁻ reduction or NH₄⁺ assimilation. Beneficial for: slowly growing cultures, embryo development (mimics seed albumin amino acid supply), recalcitrant species, and anther/microspore culture where demand for amino acid building blocks is high.
🥛 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Anther culture / microspore | 250–1000 mg/L | N&N or B5 base |
| Embryo rescue medium | 100–500 mg/L | Supplement to standard medium |
| Recalcitrant callus promotion | 100–500 mg/L | Trial range |
| Orchid seed germination | 200–400 mg/L | Historical Knudson modification |
| Suspension culture growth | 250–500 mg/L | May replace some inorganic N |
⚠️ Casein hydrolysate is a complex, undefined material — lot-to-lot variation is significant. For quantitative experiments requiring defined composition, use individual amino acids (L-glutamine, L-proline, glycine) instead.
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