Description
🌿 2-Isopentenyl Adenine (2-iP) — an intermediate-activity natural cytokinin; preferred for apple, pear, and some rose micropropagation where BAP causes fasciation or rosetting.
| 🧪 CAS | 2365-40-4 | ⚖️ MW | 203.25 g/mol |
| 🔬 Grade | TC / Research | 🌡️ Storage | −20 °C, dark |
⚗️ Activity profile
2-iP has roughly intermediate activity between kinetin and zeatin. It is naturally occurring (found in tRNA and some bacteria) and is processed by plants to trans-zeatin — making it a natural cytokinin precursor. This gives a smoother, more sustained cytokinin effect than exogenous zeatin.
🍎 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Apple (Malus) shoot multiplication | 5–20 µM (≈1–4 mg/L) | WPM base |
| Pear micropropagation | 5–15 µM | Reduces fasciation vs BAP |
| Rose shoot proliferation | 5–20 µM | MS or WPM |
| Blueberry (Anderson’s medium alt) | 5–10 µM | Some protocols prefer over 2-iP |
| Woody ornamentals | 5–25 µM | Species-specific optimisation |
🧪 Stock prep
Dissolve in 70% ethanol or DMSO. Filter-sterilise. Store at −20 °C.
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