Description
🛡️ PPM (Plant Preservative Mixture) — a proprietary broad-spectrum biocide formulation by Plant Cell Technology (USA); the most effective contamination-prevention additive for TC medium that does not harm plant cells.
| 📦 Format | Liquid concentrate, 30 mL | 🔬 Grade | TC-specific biocide |
| ⚗️ Working conc | 0.5–2 mL/L medium | 🌡️ Storage | 4 °C, sealed, dark |
🛡️ What makes PPM different from antibiotics
PPM targets bacterial and fungal mitochondria and electron transport chains — a mechanism that plant cells (with chloroplasts) are largely unaffected by. Unlike antibiotics, PPM: does not select for resistant bacteria, is effective against both gram-positive and gram-negative bacteria AND fungi, survives autoclaving intact, and has minimal effect on plant hormone signalling.
🧫 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Routine TC medium (prevention) | 0.5–1 mL/L | Add before autoclaving |
| High-risk field tissue (establishment) | 1–2 mL/L | Stage I only |
| Agrobacterium co-cultivation medium | 1–2 mL/L | After co-cultivation phase |
| Emergency contamination control | 2–3 mL/L (short-term) | 2 week max at this level |
💡 PPM is most effective as a preventive, not a curative. Add to all establishment media, especially for field-collected tissue. For already-contaminated cultures, PPM slows but may not eliminate established contamination.
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