Description
🌿 Naphthaleneacetic Acid (NAA) — the most stable synthetic auxin for TC. More persistent than IAA (not oxidised by IAA oxidase), more reliable than 2,4-D for rooting.
| 🧪 CAS | 86-87-3 | ⚖️ MW | 186.21 g/mol |
| 🔬 Grade | TC / Research | 🌡️ Storage | RT, dark, dry; stock at 4 °C |
⚗️ How it works
NAA mimics IAA at TIR1/AFB auxin receptors, promoting cell elongation, division, and root initiation. Because it is not degraded by IAA oxidase, its effect is more prolonged and predictable than natural auxin — a major advantage in a controlled TC medium.
🌱 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Root induction on shoots | 0.5–3 mg/L | ½ MS, no cytokinin |
| Callus induction (with BAP) | 0.1–2 mg/L | Higher than BAP → more callus |
| Shoot culture maintenance | 0.01–0.1 mg/L | Low conc reduces callus base |
| Adventitious root induction | 1–5 mg/L | Pulse treatment effective |
| Embryogenic callus (some spp) | 2–5 mg/L | With low cytokinin |
| Banana Stage II supplement | 0.1–0.3 mg/L | With high BAP |
🧪 Stock solution prep
Dissolve in a few drops of 1M NaOH or 70% ethanol, dilute to volume. Prepare 1 mg/mL stock, filter-sterilise, store at 4 °C.
💡 NAA at >1 mg/L without cytokinin will drive rooting in most shoot cultures. If roots appear during Stage II multiplication, reduce NAA or remove it entirely.
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