Myo-Inositol — 100g

Description

🔬 Myo-Inositol — a sugar alcohol present in every living cell; included at 100 mg/L in MS and most TC media as an essential component for cell membrane biogenesis, cell wall synthesis, and cell division signalling.

💡 Why every medium needs myo-inositol

Myo-inositol is the precursor to: phosphatidylinositol (membrane phospholipid), inositol phosphates (IP₃ — a second messenger), and cell wall pectins/hemicelluloses (galacturonosyl-inositol polysaccharides). In rapidly dividing callus and suspension cultures, the demand for these biosynthetic products far exceeds what the cell can synthesise from glucose-6-phosphate — hence the need for exogenous supplementation.

🔬 Practical points

• ✅ Standard inclusion: 100 mg/L in virtually all MS-based media
• ✅ Increase to 200–500 mg/L for high-density cell suspension or embryogenesis
• ✅ Included in Gamborg B5 vitamins mix
• ⚠️ Not a carbon source — does not replace sucrose