Description
🧫 Gamborg B5 Medium — developed by O.L. Gamborg (1968) for soybean suspension culture; the medium of choice for legumes, cell suspension, and protoplast culture.
| 📦 Format | Powder → 1 L | 🔬 Grade | TC / Tissue Culture |
| ⚗️ Working pH | 5.5–5.8 | 🌡️ Storage | 2–8 °C, sealed |
💡 Key difference from MS
B5 has lower ammonium (2 mM NH₄⁺ vs MS’s 20 mM) and a different micronutrient ratio — critical because many legumes are sensitive to high ammonium. It also includes thiamine as the only vitamin required for most suspension cultures.
🌱 Best for
| Use | Concentration | Notes |
|---|---|---|
| Soybean & legume callus | B5 + 2,4-D 1–2 mg/L | Classic protocol |
| Cell suspension culture | B5 liquid + 2,4-D | Subculture every 7 days |
| Protoplast isolation & culture | B5 modified | Low ammonium critical |
| Brassica anther culture | B5 + 0.1 mg/L BAP | Microspore development |
| Tobacco BY-2 maintenance | Modified B5 | Standard suspension medium |
💡 When adapting a legume from MS to B5, do a 50/50 blend for the first two subcultures to avoid osmotic shock.
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