Description
🍬 D-Glucose (TC Grade) — an alternative carbon source to sucrose; used when direct hexose supply is needed, in specialised culture systems, and for studying sugar signalling responses.
| 🧪 CAS | 50-99-7 | ⚖️ MW | 180.16 g/mol |
| 🔬 Grade | TC / Ultra-Pure | 🌡️ Storage | RT, sealed, dry |
💡 Glucose vs sucrose in TC
Sucrose is the preferred TC carbon source because it is the natural phloem transport sugar — plants are adapted to import it. Glucose is used when: (1) studying hexose vs sucrose signalling (different sugar sensing pathways), (2) protoplast and single-cell cultures that have low invertase activity, (3) anther culture (some protocols: pollen absorbs glucose directly), or (4) when sucrose hydrolysis during autoclaving must be avoided.
🍬 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Anther / microspore culture | 20–60 g/L glucose | Replace sucrose in some protocols |
| Protoplast culture | 10–30 g/L glucose | Osmoticum + carbon |
| Sugar signalling experiments | 0.1–100 mM | Define precisely |
| Pollen germination medium | 2–5% glucose | With boric acid + Ca²⁺ |
💡 D-Glucose undergoes Maillard browning during autoclaving — medium turns yellow-brown at high concentrations. Filter-sterilise glucose solutions >5% and add post-autoclave to prevent this.
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