Description
🍋 Citric Acid — used in TC as a mild acidifier for pH adjustment, an antioxidant chelator in some protocols, and a wash buffer component for reducing oxidative browning.
| 🧪 CAS | 77-92-9 | ⚖️ MW | 192.12 g/mol |
| 🔬 Grade | TC / Analytical | 🌡️ Storage | RT, dry, sealed |
💡 TC roles for citric acid
While not as commonly used as ascorbic acid for anti-browning, citric acid chelates metal ions (especially Cu²⁺ and Fe³⁺) that catalyse polyphenol oxidation. At low concentrations it also mildly acidifies wash solutions. Some protocols use a citric acid + ascorbic acid combination as the anti-oxidant explant wash — the chelation plus radical scavenging provides dual protection.
⚗️ Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Anti-browning wash (with ascorbic acid) | 100–300 mg/L citric acid + 150 mg/L ascorbic acid | Soak explants before culture |
| pH adjustment (mild acidifier) | Few drops 1M solution | Before autoclaving |
| EDTA chelation enhancement | 50–100 mg/L alongside EDTA | Metal sequestration |
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