Description
🌾 Chu’s N6 Medium — developed by C.C. Chu (1975, Chinese Academy of Sciences) for rice anther culture; the standard for cereal anther and monocot transformation.
| 📦 Format | Powder → 1 L | 🔬 Grade | TC / Tissue Culture |
| ⚗️ Working pH | 5.8 | 🌡️ Storage | 2–8 °C, sealed |
💡 Cereal anther culture explained
Rice (Oryza sativa) anthers placed on N6 medium at the uninucleate microspore stage can produce haploid callus that, after chromosome doubling with colchicine, yields homozygous doubled-haploid plants in one generation — compressing a 6-year inbreeding programme to 18 months. N6’s reduced ammonium and specific KNO₃:NH₄NO₃ ratio is critical for this reprogramming.
🌾 Applications
| Use | Concentration | Notes |
|---|---|---|
| Rice anther culture (callus induction) | N6 + 2,4-D 2 mg/L + kinetin 0.5 mg/L | Uninucleate microspore stage |
| Rice embryo rescue | N6 + BAP 1 mg/L | Embryo development medium |
| Wheat anther culture | N6 or N6-modified + 2,4-D | Cold pre-treatment 4°C/2d first |
| Maize callus induction | N6 + 2,4-D 1–2 mg/L | Immature embryo explant |
| Barley anther culture | N6 liquid + maltose (instead of sucrose) | Improved response |
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