Description
🍂 Abscisic Acid (ABA) — the plant stress & dormancy hormone. In TC, used for somatic embryo maturation, storage lipid accumulation, and desiccation tolerance in artificial seed protocols.
| 🧪 CAS | 14375-45-2 | ⚖️ MW | 264.32 g/mol |
| 🔬 Grade | TC / Research | 🌡️ Storage | −20 °C, strictly light-protected |
⚗️ ABA in somatic embryogenesis
After SE induction with 2,4-D or picloram, developing somatic embryos often remain in a juvenile, recalcitrant state unless matured. ABA treatment mimics zygotic embryo late maturation — triggering accumulation of storage proteins, storage lipids (triacylglycerols), and late embryogenesis abundant (LEA) proteins. This dramatically improves germination frequency and conversion to plants.
🍂 Working concentrations
| Use | Concentration | Notes |
|---|---|---|
| Somatic embryo maturation | 5–60 mg/L | 2–4 weeks on maturation medium |
| Conifer SE maturation (standard) | 7.6 mg/L (≈29 µM) | With gelrite-gelled medium |
| Desiccation tolerance induction | 1–10 mg/L | Pre-desiccation treatment |
| Stress tolerance studies | 0.1–100 µM | Dose-response experiments |
| Shoot growth inhibition (control) | 0.5–5 mg/L | Inhibits shoot elongation |
🧪 Stock prep
Dissolve in 70% ethanol or methanol. ABA is extremely light-sensitive — prepare in dim light, wrap all containers in foil immediately. Filter-sterilise, store at −20 °C foil-wrapped. Decomposes rapidly under UV/visible light.
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