Benzylaminopurine (BAP) — 1g

Description

Benzylaminopurine (BAP), also known as 6-benzylaminopurine or 6-BA, is the most widely used synthetic cytokinin in commercial plant tissue culture. It drives shoot proliferation, axillary bud break, and callus induction across a broad range of species.

Purity and Grade

Greater than 98% purity by HPLC. Suitable for research and commercial TC production work.

Preparation

BAP is not water-soluble at room temperature. Dissolve in a small volume of 1N NaOH (preferred) or DMSO, then dilute to the required volume with distilled water. Prepare a stock solution (e.g. 1mg/mL) and store refrigerated. Filter-sterilise if adding to pre-autoclaved medium, or include in medium before autoclaving — BAP is heat-stable.

Working Concentrations

Typical use range is 0.5–5.0 mg/L depending on species and developmental stage. Most multiplication media fall between 1.0–3.0 mg/L. Concentrations above 3.0 mg/L over successive subcultures increase the risk of hyperhydricity (vitrification) — shoots become water-soaked, translucent, and difficult to root. Reduce concentration or alternate with lower-cytokinin passages if this occurs.

Common Applications

Shoot proliferation in orchids, banana, ornamentals, medicinal plants, and woody species. Often used in combination with a low concentration of auxin (NAA or IBA at 0.1–0.5 mg/L) to maintain shoot architecture and reduce callus formation at the base.

Storage

Store powder at room temperature in a sealed container, away from light and moisture. Stable for at least 2 years under these conditions.